Description
| Application: | Enzyme assay, in vitro transcription For research use only! |
| Identity: | Saccharomyces cerevisiae enzyme, recombinant from yeast (Hansenula polymorpha) |
| Unit definition: | One unit is defined as the amount of enzyme necessary to hydrolyse 1 µmol diphosphate in one minute at pH 7.2 and 25 °C which is measured as phosphate released according to the Malachite Green reaction. |
| Size: | 293 amino acids; MWcalc. = 33.1 kDa (monomer) |
| Purity: | > 95 % by SDS-PAGE |
| Buffer composition: | 25 mM NaCl, 0.1 mM EDTA, 50 % v/v glycerol, 10 mM Tris-HCl, pH 7.5 (@ 25 °C) |
| Storage: | store at -20 °C, avoid freeze/thaw cycles |
| Expression system: | Hansenula polymorpha (yeast) |
| Composition: | Animal component free |
Additional information:
Inorganic Pyrophosphatase (iPPase, EC 3.6.1.1) is a recombinant hydrolase cloned from S. cerevisiae and overexpressed in H. polymorpha. The enzyme is active as dimer and requires divalent cations such as Mg 2+ to catalyse the reaction. iPPase can pull energy consuming reactions requiring ATP hydrolysis by hydrolizing the resulting diphosphate e.g. during in vitro RNA synthesis.
Coomassie stained SDS-PAGE
Lane 1: molecular weight marker; Lane 2: buffer; Lane 3: ARTES iPPase, > 95 % purity
