Description
| Application: | Specific digestion of proteins for e.g. SDS-PAGE, RP-HPLC. For research use only! |
| Identity: | Bos taurus (Bovine) enzyme, recombinant |
| Unit defintion: | 1 unit is defined as the amount of enzyme required to cleave 25 µg of a recombinant fusion protein containing the Enterokinase cleavage site to > 90 % within 18 hours at 25°C in a total reaction volume of 40 µl. |
| Size: | 241 amino acids. Calculated molecular weight 27.1 kDa |
| Purity: | > 95 % by SDS-PAGE |
| Buffer composition: | 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2, 50 % v/v Glycerol, pH 6.6 at 23 °C |
| Storage: | store at -20 °C |
| Expression system: | Hansenula polymorpha |
| Composition: | Animal component free |
Enterokinase is a specific protease that cleaves after lysine at its cleavage site “Asp-Asp-Asp-Asp-Lys”. Depending on the conformation of the protein substrate, the enzyme will sometimes cleave at other basic residues.
Recombinant Enterokinase produced in Hansenula polymorpha by ARTES.
Coomassie stained SDS-PAGE
Lane 1: molecular weight marker. Lane 2: with EK digested test substrate. Lane 3: test substrate negative control
If you need any more information at all, or if you’ve cited our amazing recombinant Endo H in a publication, please don’t hesitate to get in touch! We would absolutely love to hear from you and would be thrilled to reward your research.
